张春芳,曹秀梅,佟 琦,杨卫军,张嘉楠,杨赵伟.高效液相色谱法测定白灵菇中荧光增白剂含量[J].中国无机分析化学,2017,7(3):4-7.
高效液相色谱法测定白灵菇中荧光增白剂含量
Determination of Fluorescent Whitening Agents in Mushroom by HPLC
投稿时间:2017-03-01  最后修改时间:2017-04-10
DOI:doi:10.3969/j.issn.2095-1035.2017.03.002
中文关键词:  白灵菇  荧光增白剂  高效液相色谱
英文关键词:mushroom  fluorescent whitening agents(FWAs)  high performance liquid chromatography(HPLC)
基金项目:河北省高层次人才资助项目(A2016002037)资助
                 
作者单位
张春芳 河北省应用化学重点实验室 燕山大学环境与化学工程学院,河北 秦皇岛 066004
曹秀梅 秦皇岛市农产品质量安全监督检验中心,河北 秦皇岛 066004
佟 琦 河北省应用化学重点实验室 燕山大学环境与化学工程学院,河北 秦皇岛 066004
杨卫军 秦皇岛市农产品质量安全监督检验中心,河北 秦皇岛 066004
张嘉楠 秦皇岛市农产品质量安全监督检验中心,河北 秦皇岛 066004
杨赵伟 秦皇岛市农产品质量安全监督检验中心,河北 秦皇岛 066004
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中文摘要:
      建立了用高效液相色谱法同时测定白灵菇中3种荧光增白剂(VBL、CBS、CXT)的方法。试样用乙醇-水(V/V=1/2)溶液作提取剂,50 ℃条件下水浴静置提取15 min。采用C18(250 mm×4.6 mm,2.6 μm)为分析柱,以乙酸铵(20 mmol/L):乙腈(65%:35%)为流动相洗脱,流速为0.8 mL/min。3种荧光增白剂在0.01~10 μg/mL范围内线性关系良好,相关系数(r2)均大于0.997;加标回收率为79%~90%。方法简单准确,线性关系、重现性及加标回收率均满足分析要求。
英文摘要:
      A method for the determination of three fluorescent whitening agents (FWAs) (VBL, CBS, CXT) in mushroom by high performance liquid chromatography was developed. The sample was extracted with ethanol-water (V/V=1/2) solution by static extraction of water for 15min at 50 ℃. The HPLC method was performed on a column of ThermoFisher Accucore C18 (250 mm×4.6 mm, 2.6 μm) with elution using 20 mmol/L ammonium acetate (65%) and acetonitrile (35%) as the mobile phases with the flow rate of 0.8 mL/min. The target analytes showed good linearity in the range of 0.01—10 μg/mL with the correlation coefficients (r2) greater than 0.997. The recoveries were in the range between 79%—90%.
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