Abstract:N-N dimethylamide/nano-silver modified electrode was used to modify conductive glass (ITO) with N-N dimethylformamide and nano-silver. The electrochemical behavior of the electrode was studied. In PBS with pH 6.64 as buffer solution, metformin hydrochloride showed a good linear relationship with oxidation peak current within the concentration range of 0.075~0.2 mg/mL while linear equations was y = 317.19x + 30.07 6, and the minimum detection limit was 0.000 7 mg/ mL. The correlation linear coefficient r squared is 0.959 5. Parallel measurement of 5 sets of data with an RSD value of 0.75 per cent. In metformin hydrochloride sustained-release tablet solution, the average recovery rate was 100.96 per cent after 5 times of standard addition. The results of precision and accuracy analysis showed that this method can be applied to the determination of metformin hydrochloride in medicine.