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投稿时间：2020-01-10 修订日期：2020-03-26
投稿时间：2020-01-10 修订日期：2020-03-26
中文摘要: 建立了亲水相互作用色谱柱(HILIC)串联C18柱的方法,高效分离实际样品中的胭脂红,并对色谱柱的串联顺序以及色谱分离条件进行优化。结果表明：当HILIC柱在前C18柱在后,流动相为乙腈-5 mmol/L乙酸铵=91:9(v/v),流速为0.9 mL/min,柱温为 25 ℃,检测波长为508 nm时,此时胭脂红的分离效果最佳。在最佳的色谱分离条件下,选用两种市售饮品进行实际样品的测定,回收率在81.16 %~119.34 %之间。该方法适用于水相样品中的强极性物质的高效分离与分析。
Abstract:Carmine in real samples was separated efficiently based on affinity column tandem C18 column method. The sequence of columns in series condition was optimized. The results showed that when the mobile phase was acetonitrile-5 mmol/L ammonium acetate = 91:9 (V/V), the flow rate was 0.9 mL/min, the column temperature was 25 ℃, the detection wavelength was 508 nm, and the column in series sequence was HILIC column tandem C18 column, the separation effect of carmine was the best. Under the optimal chromatographic conditions, the commercial beverage plum garden strawberry flavor milk was analyzed and carmine was found in the sample. And the recovery was between 105.15% and 113.28%. The proposed method is suitable for the analysis of strongly polar compounds in water samples.
文章编号： 中图分类号：O657.7 文献标志码：
基金项目:国家自然科学(21565018); 云南省重点研发计划项目(2018BC005-04); 云南中烟工业有限责任公司重点项目(2018JC05); 昆明理工大学大学生课外学术科技创新基金(2018BA387)资助; 昆明理工大学分析测试(S2017M20162111056、2017M20152111050).
引用文本：
刘智敏,余诗雨,刘育坚,许志刚,刘宏程,司晓喜.亲水相互作用色谱柱串联C18柱高效分析饮品中的胭脂红[J].中国无机分析化学,2020,10(3):14-17.
Liuzhimin,Yushiyu,Liuyujian,xuzhigang,Liuhongcheng,Sixiaoxi.Separation of carmine in beverages by affinity column and C18 column high performance liquid chromatography[J].Chinese Journal of Inorganic Analytical Chemistry,2020,10(3):14-17.
刘智敏,余诗雨,刘育坚,许志刚,刘宏程,司晓喜.亲水相互作用色谱柱串联C18柱高效分析饮品中的胭脂红[J].中国无机分析化学,2020,10(3):14-17.
Liuzhimin,Yushiyu,Liuyujian,xuzhigang,Liuhongcheng,Sixiaoxi.Separation of carmine in beverages by affinity column and C18 column high performance liquid chromatography[J].Chinese Journal of Inorganic Analytical Chemistry,2020,10(3):14-17.
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