Abstract:In order to accurately quantify the trace elements in the ant, the samples were pretreated by microwave digestion with HNO3-H2O2 mixed acid as the digestion system.The appropriate isotopes were selected. 52Cr、55Mn、60Ni、63Cu、66Zn、75As、78Se、88Sr and 114Cd were determined with 103Rh as internal standard, and 202Hg and 208Pb were determined with 185Re as internal standard. A method for the determination of 11 trace elements in Polyrhachis vicina rogers by inductively coupled plasma mass spectrometry was established. It was found that the sample was completely digested with HNO3-H2O2 as the digestion system. Ked mode was used to eliminate the interference of mass spectrometry, and the matrix effect was corrected by internal standard method. By drawing the calibration curve and determining the blank of the process, the correlation coefficient of the calibration curve of each element was greater than 0.999, the detection limit of the method was 0.0005 -0.0109 mg /kg, and the quantitative limit of the method was 0.0016 -0.0327 mg/ kg.The actual samples of Polyrhachis vicina rogers were determined. The relative standard deviations (RSD, n = 9) were in the range of 1.19 %-5.47 %, and the recoveries were 91.2 %-107.0 %. Through the comparison test between laboratories, the relative deviations were between - 3.15 % and 2.74 %. The samples of Polyrhachis vicina roger from six different habitats were determined. The results showed that there were great differences in the contents of other 10 trace elements except cadmium in samples from different habitats.The results of this method were stable and reliable, and could meet the requirements for the analysis and detection of 11 trace metal elements in Polyrhachis vicina roger.