Abstract:A sensitizer with a suitable proportion will have a certain optimization effect on the determination of selenium content in food by super microwave digestion-inductively coupled plasma mass spectrometry. In this paper, the super microwave digestion instrument was used to fully digest 1.0 g food samples, the collision cell mode was used to avoid mass spectrometry interference, the internal standard was corrected for non-mass spectral interference, methanol, ethanol, acetic acid, isopropanol, and glycerol were selected as sensitizers, the addition ratio during the test process was adjusted, as well as inductively coupled plasma mass spectrometry was used. The results showed that the response value of selenium was the highest in the presence of 2% isopropanol, and the method limit of detection was 0.0003 mg·kg-1. The above methods were used to analyze food standard substances (GSB-8, GSB-9, GSB-24, GSB-30) and actual samples; the measured values of the food standard substances were within the range of their identified values and uncertainties, and the relative error was less than -5,4%. The relative standard deviation (n=7, RSD) of the actual sample measurement value was 0.3~1.7%, and the recovery rate of spiking was 99.2~105%. The method is simple, precise and accurate, and can be used for the determination of selenium content in various food samples.