Abstract:The experiment aimed to study the enrichment and distribution of selenium in Sanhuang Chicken supplemented with different types and doses of selenium-containing supplements to provide a reference for the development of selenium-enriched meat. Determination of total selenium in the tissues of Sanhuang chickens by inductively coupled plasma mass spectrometry with microwave digestion, which was fed with sodium selenite, selenium-enriched Cardamine violifolia, and selenium-enriched yeast. The selenium species were isolated from both muscle and liver tissues through ultrasound-assisted enzymatic digestion performed at 55 ℃ in a water bath. Following separation on a ZORBAX SB-Aq C18 chromatographic column, the analysis was carried out using a mobile phase consisting of 10 mmol/L sodium citrate + 5 mmol/L sodium hexane sulfonate (containing 1% methanol, pH=4.0). The four selenium species, selenate(Se(VI), selenocysteine (SeCys), methylselenocysteine (MeSeCys), and selenomethionine (SeMet), were analyzed and quantified by inductively coupled plasma mass spectrometry coupled with high-performance liquid chromatography (HPLC-ICP/MS). The results showed that the selenium content in the muscles and organs of the three groups was highest in the selenium-enriched yeast group. In chicken breast and thigh muscles, the main selenium species is SeMet, while in the liver, SeCys is the primary selenium form. Tissues from the group fed with selenium-enriched yeast exhibited a significantly higher content of SeMet compared to the other groups. The research findings indicate that the type of selenium supplement added has an impact on the selenium enrichment in chicken tissues. Feeding with selenium-enriched yeast significantly increases the selenium content in livers and muscles, particularly the content of SeMet. This information can serve as a reference for the feeding of selenium-enriched broilers.