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| Determination of Trace Hydrogen Peroxide by Fluorescence Quenching Method of Glutathione-capped CdTe Quantum Dots |
| Received:November 22, 2011 Revised:March 31, 2012 |
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| DOI：doi:10.3969/j.issn.2095-1035.2012.02.0003 |
| KeyWord:glutathione;CdTe quantum dots;hydrogen peroxide;fluorescence quenching |
| Author | Institution |
| CHEN Zhibing |
宿州学院 化学与生命科学学院，安徽 宿州 |
| CHA Jun |
宿州学院 化学与生命科学学院，安徽 宿州 |
| SONG Qian |
宿州学院 化学与生命科学学院，安徽 宿州 |
| LIU Xin |
宿州学院 化学与生命科学学院，安徽 宿州 |
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| Abstract: |
| By using glutathione-capped CdTe quantum dots (QDs) as a fluorescent (PL) probe, a new fluorescence quenching method was developed to quantitatively determine hydrogen peroxide based on PL quenching effect of CdTe QDs. The influences of buffer solution, QDs concentration and incubation time on the fluorescence behavior of QDs were investigated. The results indicated that in a Na2HPO4-NaH2PO4 buffer solution at pH=7.2 and with 15 min incubation, the determined concentration of hydrogen peroxide was in the ranges of 1.0×10-6～3.0×10-5 mol/L. The fluorescence intensity of CdTe QDs and the concentration of hydrogen peroxide follow the linearly fitted equation as: △F = 9.38+7.56c (10-6 mol/L) with the correlation coefficient of 0.9992. The detection limit was 1.27×10-8 mol/L. The fluorescence quenching method of glutathione-capped CdTe QDs has been applied to analyze water samples. The recoveries were between 96% to 103% and relative standard deviation of the method was less than 3.3%, which were satisfactory. |
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