Ion Chromatography Determination of Contents of Captopril and Hydrochlorothiazide in Compound Captopril Tablets
Received:July 06, 2012  Revised:July 31, 2012
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DOI:doi:10.3969/j.issn.2095-1035.2012.03.023
KeyWord:ion chromatography; compound captopril tablets; captopril; hydrochloro-thiazide
        
AuthorInstitution
LV Weidei 杭州职业技术学院,杭州
ZHOU Dan 桂林理工大学 化学与生物工程学院,桂林
ZHU Yan 浙江大学 化学系,杭州
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Abstract:
      An analytical method for quantitative determination of captopril and hydrochlorothiazide in compound captopril tablets by ion chromatography(IC) was established. The method was performed on IonPac SCS1(4 mm×250 mm) chromatographic column and IonPac SCG1(4 mm×50 mm) guard column. The chromatographic conditions were: the mixture of 4 mmol/L methanesulfonic acid solution and 26% acetonitrile was used as the mobile phase; the flow rate was 1 mL/min, the column temperature was 35 ℃; the injection volume was 7 μL, and the detection wavelength was 215 nm. The results showed that the calibration curves of captopril and hydrochlorothiazide had good linearity in the range of 0.5~25 μg/m with the correlation coefficient of 0.9997 and 0.9999, respectively. The average recovery for captopril and hydrochlorothiazide was 98.11 % and 94.24 %, respectively. The limit of detection for captopril and hydrochlorothiazide was 0.012 and 0.002 μg/mL, respectively. The method was proved to be simple, sensitive, accurate, and reliable for evaluating the quality of compound captopril tablets.
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