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| Determination of Nitrite in Blood by Ion Chromatography |
| Received:July 02, 2012 Revised:July 18, 2012 |
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| DOI：doi:10.3969/j.issn.2095-1035.2013.01.020 |
| KeyWord:ion chromatography;nitrite;blood;conductivity detection |
| Author | Institution |
| WANG Yong |
南京市公安局 刑事科学技术研究所，江苏 南京 |
| ZUO Yuexian |
南京市公安局 刑事科学技术研究所，江苏 南京 |
| SONG Shengli |
重庆市公安局 物证鉴定中心，重庆 |
| HUO Zongli |
江苏省疾病预防控制中心，江苏 南京 |
| CHAI Jinfeng |
山西省河津市刑警大队，山西 运城 |
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| Abstract: |
| A method for the determination of nitrite in blood by ion chromatography was established. The protein in blood was precipitated with acetonitrile and then the large molecules and Cl- in the supernatant were removed by using a Dionex OnGuard Ⅱ RP column and a Dionex OnGuard Ⅱ Ag column. The filtrate went through gradient elution with KOH solution produced by an online eluent generator(EG). The suppressor took the external water mode and a conductivity detector was used. When the concentration of NO2- standard solution are in the range of 0.214~21.4 mg/L, the concentration has a good linear relationship with detection signal and the linear equation is Y=0.0209X+0.5189 with r2=0.9999. The limit of detection (LOD,S/N=3) of nitrite in blood was 0.39 μg/L. The average recoveries were between 96.5% to 101.2%. Because of fast testing, easy instrumental operation, high recovery, good repeatability, this method can meet the needs for public security work. |
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