Study on Trace Hydrogen Sulfide Artificial Enzyme Potentiometric Biosensor
Received:March 28, 2016  Revised:August 29, 2016
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DOI:doi:10.3969/j.issn.2095-1035.2016.04.019
KeyWord:hydrogen sulfide; open circuit potential; electrochemical biologic mimic enzyme biosensor; electrochemical sensor
                 
AuthorInstitution
ZHAO Junjian 沈阳师范大学 化学化工学院,沈阳
HAN Zhixuan 沈阳师范大学 化学化工学院,沈阳
YANG Xiuhui 沈阳师范大学 化学化工学院,沈阳
HAN Xiaowei 沈阳师范大学 化学化工学院,沈阳
SUN Zhaoqi 沈阳师范大学 化学化工学院,沈阳
ZHU Yongchun 1沈阳师范大学 化学化工学院,沈阳 ;2沈阳师范大学 化学化工学院 能源与环境催化研究所,沈阳
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Abstract:
      In the present work, a single layer of zinc film was galvanized on the carbon fiber cluster electrode by potentiostatic method in alkaline aqueous solution with pH=9.0. In the presence of the coating layer of histidine and electrolytic zine oxide, a zinc-histine-hydroxyl complex modified electrode was in-situ prepared and used to mimic the process in which hydrogen sulfide ions are recognized by biological enzyme molecules. The experimental conditions of the modified electrode preparation method were optimized. An open circuit potential method for the determination of trace hydrogen sulfide was developed based on this modified electrode. In neutral aqueous solution, the open circuit potentials at the modified electrode had regular changes with the concentrations of the hydrogen sulfide ions which were injected into the solution. And the relationship between open circuit potentials and hydrogen sulfide ion concentration can be described by Nernst equation. The method was used to determine the concentration of sulfide in aqueous solution in the range of 1.0×10-6-1.0×10-15 mol/L, with its lowest detection limit of 1.0×10-15 mol/L. The relative standard deviation was 3.5% with the recovery of 109.2%. The advantages, including fast response speed, high sensitivity, low detect limits, wide detection range and easy operation, made the proposed method suitable for the determination of the concentration of hydrogen sulfide ions in river water.
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