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| Determination of Fluorescent Whitening Agents in Mushroom by HPLC |
| Received:March 01, 2017 Revised:April 10, 2017 |
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| DOI：doi:10.3969/j.issn.2095-1035.2017.03.002 |
| KeyWord:mushroom; fluorescent whitening agents(FWAs); high performance liquid chromatography(HPLC) |
| Author | Institution |
| ZHANG Chunfang |
河北省应用化学重点实验室 燕山大学环境与化学工程学院，河北 秦皇岛 |
| CAO Xiumei |
秦皇岛市农产品质量安全监督检验中心，河北 秦皇岛 |
| TONG Qi |
河北省应用化学重点实验室 燕山大学环境与化学工程学院，河北 秦皇岛 |
| YANG Weijun |
秦皇岛市农产品质量安全监督检验中心，河北 秦皇岛 |
| ZHANG Jianan |
秦皇岛市农产品质量安全监督检验中心，河北 秦皇岛 |
| YANG Zhaowei |
秦皇岛市农产品质量安全监督检验中心，河北 秦皇岛 |
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| Abstract: |
| A method for the determination of three fluorescent whitening agents (FWAs) (VBL, CBS, CXT) in mushroom by high performance liquid chromatography was developed. The sample was extracted with ethanol-water (V/V=1/2) solution by static extraction of water for 15min at 50 ℃. The HPLC method was performed on a column of ThermoFisher Accucore C18 (250 mm×4.6 mm, 2.6 μm) with elution using 20 mmol/L ammonium acetate (65%) and acetonitrile (35%) as the mobile phases with the flow rate of 0.8 mL/min. The target analytes showed good linearity in the range of 0.01—10 μg/mL with the correlation coefficients (r2) greater than 0.997. The recoveries were in the range between 79%—90%. |
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