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| Determination of Zinc in Abutilon Fruit by Double-Wavelength Spectrophotometry |
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Received:October 14, 2016
Revised:December 11, 2016
Published Online:September 30, 2017
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| DOI：doi:10.3969/j.issn.2095-1035.2017.03.019 |
| KeyWord:xylenol orange；double-wavelength; zinc ; Abutilon |
| Author | Institution |
| CHEN Xiaohong |
College of Chemical Technology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia , China |
| DING Wenwen |
College of Chemical Technology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia , China |
| XU Ling |
College of Chemical Technology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia , China |
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| Abstract: |
| A colored coordination complex was formed by Zn2 + and xylenol orange in pH 6.0 hexamethylenetetramine buffer solution. The color faded at 570 nm and formed at 430 nm. The results show that △A=A440－A570 is linearly related to the Zn2 + concentration within certain range. The double-wavelength spectrophotometry for the determination of Zn2 + content is established. Lambert-Bill laws were implemented when the Zn2 + concentration ranged from 0.1 to 0.6 μg/mL. The detection limit was 1.5×10-2 μg/mL. The method, which is simple and accurate, has been applied to the determination of zinc in Abutilon fruit. The relative standard deviation was less than 5% and the recovery was between 95.0% and 96.0%. |
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