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| Separation of carmine in beverages by affinity column and C18 column high performance liquid chromatography |
| Received:January 10, 2020 Revised:March 26, 2020 |
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| DOI：doi:10.3969/j.issn.2095-1035.2020.03.003 |
| KeyWord:column tandem; hHydrophilic interaction column; C18 column; beverages; carmine |
| Author | Institution |
| Liuzhimin |
昆明理工大学 |
| Yushiyu |
昆明理工大学 |
| Liuyujian |
昆明理工大学 |
| xuzhigang |
昆明理工大学 |
| Liuhongcheng |
云南省农业科学院质量标准与检测技术研究所 |
| Sixiaoxi |
云南中烟工业有限责任公司技术中心;云南中烟工业有限责任公司技术中心 |
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| Abstract: |
| Carmine in real samples was separated efficiently based on affinity column tandem C18 column method. The sequence of columns in series condition was optimized. The results showed that when the mobile phase was acetonitrile-5 mmol/L ammonium acetate = 91:9 (V/V), the flow rate was 0.9 mL/min, the column temperature was 25 ℃, the detection wavelength was 508 nm, and the column in series sequence was HILIC column tandem C18 column, the separation effect of carmine was the best. Under the optimal chromatographic conditions, the commercial beverage plum garden strawberry flavor milk was analyzed and carmine was found in the sample. And the recovery was between 105.15% and 113.28%. The proposed method is suitable for the analysis of strongly polar compounds in water samples. |
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