|
| Determination of Inorganic Mercury, Methyl Mercury, Ethyl Mercury in Chinese Herbal Medicine by HPLC-ICP-MS |
| Received:August 30, 2022 Revised:September 06, 2022 |
| View Full Text View/Add Comment Download reader |
| DOI：doi:10.3969/j.issn.2095-1035.2023.05.002 |
| KeyWord:Inorganic mercury; Methyl mercury; Ethyl mercury; HPLC-ICP-MS; Chinese herbal medicine. |
| Author | Institution |
| MENG Shan |
河北省药品医疗器械检验研究院 |
| LIU Xiaoming |
河北省药品审评中心 |
| PING Huijuan |
河北省药品医疗器械检验研究院 |
| DUAN Qiong |
河北省药品医疗器械检验研究院 |
| FENG Shuhua |
河北省药品医疗器械检验研究院 |
| LIU Yongli |
河北省药品医疗器械检验研究院 |
|
| Hits: 1538 |
| Download times: 670 |
| Abstract: |
| Objective HPLC-ICP-MS was used to establish the method of simultaneous determination of inorganic mercury, methyl mercury, ethyl mercury in Chinese herbal medicine. To accumulate basic data and provide technical support for Chinese medicine yinpian quality safety regulation, the content of 106 batches of 3 different forms of mercury compounds in Chinese herbal medicine were determined. Methods The sample was extracted by artificial gastric juice in the water bath heating. Methanol-ammonium acetate as the mobile phase, using C18 reversed-phase chromatographic column separation of sample solution, finally the content of three different forms of mercury compounds were determined by ICP-MS. Results The results of three different forms of mercury compounds in 0.5 ~ 5 ng/mL range are good linear relationship, and the correlation coefficient were greater than 0.999.To evaluate the accuracy by the method of standard addition recovery, the recovery is 75.5% ~ 118.0%, RSD was 2.4% ~ 9.7%. In 106 batches of Chinese herbal medicine, methyl mercury and ethyl mercury have not been detected. The detected rate of inorganic mercury was 88.7%, and the percent of pass is 100%. Conclusion The method can accurately and efficiently determinate of the content of three different forms of mercury compounds in Chinese herbal medicine. The main mercury compound of 106 batches in Chinese herbal medicine is inorganic mercury as the existence form. |
| Close |
|
|
|