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| Determination of 8 Heavy Metals and Quasi Metal Elements in Blood and Urine by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) with KED Collision Mode |
| Received:July 12, 2023 Revised:December 22, 2023 |
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| DOI：10.3969/j.issn.2095-1035.2024.04.003 |
| KeyWord:ICP-MS; Standard addition method; Heavy metals; Quasi-metals; Blood; Urine |
| Author | Institution |
| WU Ze-qing |
中国人民公安大学 侦查学院 |
| ZHANG Cheng-long |
中国人民公安大学 侦查学院 |
| GUO Fei |
淄博市公安局刑侦支队 |
| LIU Fan-fan |
淄博市公安局刑侦支队 |
| BI Wen-ji |
淄博市公安局刑侦支队 |
| YANG Rui-qin |
中国人民公安大学 侦查学院 |
| LI Peng |
淄博市公安局刑侦支队 |
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| Abstract: |
| In metal poisoning and death cases, the accuracy of the standard curve method commonly used in the laboratory for quantitative analysis of heavy metals and quasi-metallic elements in the human body is susceptible to the differences in the matrix of blood and urine of different populations as well as matrix effects, for this reason, a method for the determination of 8 heavy metals and quasi-metallic elements, including Cr, Co, Ni, As, Cd, Sb, Pb and Tl, in human blood and urine by direct dilution-inductively coupled plasma mass spectrometry (ICP-MS) coupled with a standard addition method has been established. The collision gas flow rate and carrier gas flow rate were optimized to improve the sensitivity for the qualitative and quantitative determination of the 8 target elements. The effects of the ICP-MS sensitizer Triton X-100 and ethanol on the elemental response values at different volume fractions were investigated, and the sample was diluted 50 times using a 0.1% HNO3-0.05% Triton X-100 mixture solution as blood diluent, and 0.1% HNO3 -1% ethanol solution was used as the urine diluent to dilute the samples 10 times, which improved the response values of the elements in the sample solution and avoided the influence of the undissolved organic matter on the signal of the instrument. The application of the standard addition method avoids the introduction of other blank samples to quantify the target elements, maintains the consistency of the matrix in the quantification process and thus overcomes the matrix effect in the samples, adopts the Kinetic Energy Discrimination (KED) mode for the determination of the target elements after direct dilution, the interfering ions collision with He cleavage effectively reduces the polyatomic ion interference, and selects the cleaning time of 60 s to reduce the memory effect of the elements. The limits of detection (LOD) of the method were 0.0060-0.0630 μg/L for each element, and the limits of quantification (LOQ) were 0.0200-0.2100 μg/L. The recoveries in blood and urine were 78.85%-117.88%, and the intra- and inter-day precisions were both less than 8.7%. The established method is fast, accurate, sensitive and safe to operate, and the method and related data can provide methodological reference and data support for the testing and identification of heavy metals and quasi-metallic elements in blood and urine samples in related cases. |
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