Abstract:In recent years, complex multi-element bioenrichment studies have garnered significant attention. However, systematic research on the multi-element enrichment capacity of the Se hyperaccumulator Cardamine hupingshanensis remains limited. This paper aims to explore the multiple elemental enrichment capacities and potential applications of the hyperaccumulator Cardamine hupingshanensis. Specimens collected from the Yutangba selenium orefield were tested using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). This test provided comprehensive data on the distribution of multiple elements within various plant tissues. And the data were analyzed comprehensively using mean value comparison analysis, translocation factor, and element correlation analysis methods. The study revealed that Cardamine hupingshanensis exhibits a remarkable hyperaccumulation ability for Se, Zn, Cd, and Mo. Notably, Se is uniformly enriched throughout the entire plant, whereas Zn and Cd are predominantly concentrated in the leaves, and Mo is concentrated in the roots and leaves. The first three enriched elements (Se, Zn, and Cd) also demonstrate a strong upward mobility and transport efficiency within the plant, while Mo is more likely to be present in the root. Furthermore, the detected elements can be classified into two distinct categories based on their interactions: Mo, Se, and Cr form one group, while Ag and Zn constitute another, but it does not support classifying the elements Cd and Co into the aforementioned two categories due to their complex interaction relationship with other elements. Antagonistic interactions were observed between elements from different groups, whereas elements within the same group exhibited synergistic relationships. However, these interactions may be varied across different plant tissues, with even some element combinations displaying opposing relationships in different organs. For instance, in the root, Zn and Cu were significantly strongly positively correlated, while in the leaf, Zn and Cu were strongly negatively correlated. This may be related to multiple factors such as the concentration, types, and interrelationships of various elements within the plant, as well as the differences in the transport mechanisms of elements from soil to the plant and among different parts of the plant. In summary, the data we have detected in this study is the result of the interaction mechanisms among various elements that Cardamine hupingshanensis produced under the high concentration of Se element stress in the field. Given its ability to hyperaccumulate multiple elements, Cardamine hupingshanensis holds significant potential for diverse applications, including Se extraction, Se deposit exploration, and ecological restoration. Aligned with the requirements of regional progress, these applications offer promising prospects for local economic development and environmental sustainability.