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| Rapid determination of soil inorganic nitrogen by a microplate reader with colorimetric method |
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Received:November 14, 2018
Revised:December 11, 2018
Accepted:December 11, 2018
Published Online:May 27, 2019
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| DOI：doi:10.3969/j.issn.2095-1035.2019.02.008 |
| KeyWord:ammonium nitrogen; nitrate nitrogen; microplate reader; soil |
| Author | Institution |
| SONG Shuhui |
Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing |
| WANG Rujian |
Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing |
| XIN Yu |
Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing |
| ZHANG Jinyao |
Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing |
| WANG Hong |
Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing |
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| Abstract: |
| It is important to quickly and accurately determine the ammonium and nitrate content in the soil for assessing soil fertility and ecological environment and for directing fertilizer recommendation. In this paper, the contents of NH4+-N、NO3--N in soil were determined through a microplate reader with indophenol-blue colorimetric method and barium sulfate reduction method, respectively. We selected 30 soil samples for determination and compared with that of AA3 in order to establish method for soil inorganic nitrogen determination. The results of NH4+-N and NO3--N determined by the microplate reader and the continuous flow analyzer were compared. Regression equation for NH4+-N was established as Y(Continuous flow analyzer- NH4+-N)=0.9976X (Microplate Reader-NH4+-N)-0.0123with correlation coefficient r=0.9619(n=30,P＜0.01). Regression equation for NO3--N was Y(Continuous flow analyzer-NO3--N)=0.9593X (Microplate Reader-NO3--N)+0.0219 with correlation coefficient r=0.9640(n =30,P＜0.01). The recovery rates of NH4+-N and NO3--N concentrations measured by the microplate reader were 96.21%~107.95% and 94.91%~110.04%, respectively. The relative standard deviation (RSD) of proposed colorimetric method with microplate reader was less than 10% and 5% (n=6) for NH4+-N and NO3—N, respectively. The detection limits of NH4+-N and NO3--N content by the microplate reader were 0.068 mg/L and 0.028 mg/L, respectively. It is suggested that microplate reader with colorimetric method can be used for rapid analysis of ammonium and nitrate nitrogen content in soil extracts with high accuracy and precision. |
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